Plasmid

Part:BBa_K3861005:Design

Designed by: Kristin Funke   Group: iGEM21_Humboldt_Berlin   (2021-10-01)


pKF01


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 997
    Illegal XhoI site found at 2804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequences interfering with RCF10 or RCF25 standard were mutated silently considering the codon usage of Salmonella Typhimurium, thus no amino acids were changed.


Source

Inserted CDSs taken from Salmonella Typhimurium ATCC14028 [1]

References

[1] NCBI Reference Sequence: NZ_CP034230.1